Preparation of Siliconized Microcentrifuge Tubes
Biotech Glossary |
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Lab Protocol |
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Malaysia University |
Preparation of Siliconized Microcentrifuge Tubes
Contributor:
The Laboratory of Giovanna Ferro-Luzzi Ames at the University of California, Berkeley
Overview
This protocol describes a method of treating tubes that prevents the absorption of nucleic acids to the tube wall. Siliconized plasticware and glassware is particularly helpful when working with small amounts of single stranded DNA.
Procedure
1. Sterilize microcentrifuge tubes by autoclaving for 20 minutes.
2. Allow tubes to cool to room temperature.
3. Fill a Pasteur pipette with Sigmacote™.
4. Dispense Sigmacote™ into a sterile microcentrifuge tube (see Hint #1).
5. Using the same Pasteur pipette, remove all of the Sigmacote™.
6. Continue this procedure until as many microcentrifuge tubes as desired have come in contact with the Sigmacote™.
7. Return the remaining Sigmacote™ to the original bottle and store as described by the manufacturer.
8. Rinse each microcentrifuge tube with sterile ddH2O.
9. Place the microcentrifuge tubes in a drying oven for approximately 30 min (or until completely dry).
Solutions
This bioProtocol does not use any solutions
BioReagents and Chemicals
Sigmacote™
Protocol Hints
1. Confirm that the Sigmacote™ reaches all the way to the top of the microcentrifuge tube.
